Cytokeratin (LMW) anticorps (CF®647)

N° du produit ABIN6162923

Cet anticorps anti-Cytokeratin (LMW) Monoclonal Souris conjugué à CF®647 (Clone AE-1) (ABIN6162923) détecte spécifiquement Cytokeratin (LMW) dans WB, FACS, IF et IHC (f). L’anticorps est réactif avec des échantillons de Humain, Rat, Poulet, Boeuf (Vache), Singe, Souris, Lapin, Chien et Tortue.

Aperçu rapide pour Cytokeratin (LMW) anticorps (CF®647) (ABIN6162923)

Antigène: Cytokeratin (LMW)

Reactivité: Humain, Rat, Poulet, Boeuf (Vache), Singe, Souris, Lapin, Chien, Tortue

Hôte: Souris

Clonalité: Monoclonal

Conjugué: CF®647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: AE-1

Détail du produit

Fonction: Mouse Monoclonal anti-Cytokeratin, LMW (AE-1), CF647 Conjugate

Attributs du produit: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human epidermal keratin

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Skin, Squamous cell carcinoma (SCC)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détail du antigène

Antigène: Cytokeratin (LMW)

Autre désignation: Cytokeratin, LMW

Sujet: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Poids moléculaire: 40-56.5 kDa

ID gène: 374454, 334989

UniProt: Q7Z794