Involucrin anticorps (Biotin)

N° du produit ABIN6164806

L’anticorps Souris Monoclonal anti-Involucrin a été validé pour IF, FACS, ISt et IHC (f). Il convient pour détecter Involucrin dans des échantillons de Humain.

Aperçu rapide pour Involucrin anticorps (Biotin) (ABIN6164806)

Antigène: Involucrin (IVL)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est conjugé à/à la Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: IVRN-827

Détail du produit anti-Involucrin anticorps (Biotin)

Fonction: Mouse Monoclonal anti-Involucrin (IVRN/827), Biotin Conjugate

Attributs du produit: This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Purified involucrin from human keratinocytes

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37C
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Note than enzyme digestion is better than citrate buffer based epitope unmasking
• Optimal dilution for a specific application should be determined by user

Commentaires:

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: Involucrin

Sujet: IVL

Poids moléculaire: 66-170 kDa

ID gène: 3713, 516439

UniProt: P07476