TIMP2 anticorps (N-Term) (CF®405S)

N° du produit ABIN6165461

Cet anticorps anti-TIMP2 est un anticorps Souris Monoclonal détectant TIMP2 dans WB, IHC, IF et FACS. Adapté pour Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin et Cobaye.

Aperçu rapide pour TIMP2 anticorps (N-Term) (CF®405S) (ABIN6165461)

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIMP2 est conjugé à/à la CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 3A4

Détail du produit anti-TIMP2 anticorps (CF®405S)

Épitope: AA 56-66, N-Term

Fonction: Mouse Monoclonal anti-TIMP2 (3A4), CF405S Conjugate

Attributs du produit: This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur TIMP2

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Autre désignation: TIMP2

Sujet: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2

Poids moléculaire: 21 kDa

ID gène: 7077, 633514

UniProt: P16035

Pathways: cAMP Metabolic Process