ICAM-3/CD50 anticorps (CF®640R)
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- Antigène Voir toutes ICAM-3/CD50 (ICAM3) Anticorps
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ICAM-3/CD50 est conjugé à/à la CF®640R
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-CD50 (ICAM-3) (186-2G9), CF640R Conjugate
- Attributs du produit
- This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Stimulated human leukocytes
- Clone
- 186-2G9
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ICAM3 Anticorps primaire
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- Indications d'application
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Blocks binding of CD11a LFA-1 to CD50 ICAM-3
- Optimal dilution for a specific application should be determined by user
- Commentaires
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HL-60 or THP-1 cells. Lymph node and tonsil
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Autre désignation
- CD50 (ICAM-3) (ICAM3 Produits)
- Synonymes
- anticorps CD50, anticorps CDW50, anticorps ICAM-R, anticorps ICAM-3, anticorps ICAM3, anticorps intercellular adhesion molecule 3, anticorps ICAM3, anticorps Icam3
- Sujet
- ICAMR, Intercellular adhesion molecule 3 (ICAM3)
- Poids moléculaire
- 110-160 kDa
- ID gène
- 3385, 354563
- UniProt
- P32942
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