ICAM-3/CD50 anticorps (CF®594)

N° du produit ABIN6169458

Cet anticorps Souris Monoclonal détecte spécifiquement ICAM-3/CD50 dans FACS, IF et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour ICAM-3/CD50 anticorps (CF®594) (ABIN6169458)

Antigène: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM-3/CD50 est conjugé à/à la CF®594

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 186-2G9

Détail du produit anti-ICAM-3/CD50 anticorps (CF®594)

Fonction: Mouse Monoclonal anti-CD50 (ICAM-3) (186-2G9), CF594 Conjugate

Attributs du produit: This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Stimulated human leukocytes

Isotype: IgG2b kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Blocks binding of CD11a LFA-1 to CD50 ICAM-3
• Optimal dilution for a specific application should be determined by user

Commentaires:

HL-60 or THP-1 cells. Lymph node and tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur ICAM-3/CD50

Antigène: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Autre désignation: CD50 (ICAM-3)

Sujet: ICAMR, Intercellular adhesion molecule 3 (ICAM3)

Poids moléculaire: 110-160 kDa

ID gène: 3385, 354563

UniProt: P32942