Keratin 10 anticorps (CF®647)

N° du produit ABIN6172368

Cet anticorps Souris Monoclonal détecte spécifiquement Keratin 10 dans FACS, IF et IHC (f). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Keratin 10 anticorps (CF®647) (ABIN6172368)

Antigène: Keratin 10 (KRT10)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 10 est conjugé à/à la CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT10-844

Détail du produit anti-Keratin 10 anticorps (CF®647)

Fonction: Mouse Monoclonal anti-Cytokeratin 10 (KRT10/844), CF647 Conjugate

Attributs du produit: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human KRT10 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

A431, HeLa, MCF7 cells. Esophagus or tonsil.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Keratin 10

Antigène: Keratin 10 (KRT10)

Autre désignation: Cytokeratin 10

Sujet: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Poids moléculaire: 56.5 kDa

ID gène: 3858, 99936

UniProt: P13645