MLANA anticorps (Biotin)

N° du produit ABIN6173187

Cet anticorps Souris Monoclonal détecte spécifiquement MLANA dans WB, IF, FACS, ISt et IHC (f). Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour MLANA anticorps (Biotin) (ABIN6173187)

Antigène: MLANA (Melan A (MLANA))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLANA est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: A103

Détail du produit anti-MLANA anticorps (Biotin)

Fonction: Mouse Monoclonal anti-MART-1 / Melan-A (A103), Biotin Conjugate

Attributs du produit: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant hMART-1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur MLANA

Antigène: MLANA (Melan A (MLANA))

Autre désignation: MART-1 / Melan-A

Sujet: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

Poids moléculaire: 20-22 kDa (doublet)

ID gène: 2315, 154069

UniProt: Q16655