CD16 anticorps (CF®594)

N° du produit ABIN6173562

Détail du produit anti-CD16 anticorps (CF®594)

Antigène: CD16

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est conjugé à/à la CF®594

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: Mouse Monoclonal anti-CD16 (C16/1045), CF594 Conjugate

Attributs du produit: This antibody recognizes CD16 (FcγRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (FcγRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcγRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human CD16 protein

Clone: C16-1045

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

K-562 or U-937 cells. Lymph nodes and tonsils.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur CD16

Antigène: CD16

Autre désignation: CD16 (CD16 Produits)

Synonymes: anticorps Fcgr3, anticorps Fcgr3a, anticorps CD16, anticorps FCGR3, anticorps FCGR3@, anticorps CD16b, anticorps FCG3, anticorps FCR-10, anticorps FCRIII, anticorps FCRIIIb, anticorps FcRIII, anticorps Fc fragment of IgG, low affinity IIa, receptor, anticorps Fc fragment of IgG, low affinity IIIa, receptor (CD16a), anticorps Fc fragment of IgG receptor IIIb, anticorps Fc fragment of IgG receptor IIIa, anticorps Fc receptor, IgG, low affinity III, anticorps Fc-gamma RIII, anticorps Fcgr2a, anticorps FCGR3A, anticorps FCGR3B, anticorps Fcgr3, anticorps FCGR3

Poids moléculaire: 50-80 kDa

ID gène: 2214, 372679

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process