kappa Light Chain anticorps (CF®488A)

N° du produit ABIN6173940

Cet anticorps Souris Monoclonal détecte spécifiquement kappa Light Chain dans FACS, WB et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour kappa Light Chain anticorps (CF®488A) (ABIN6173940)

Antigène: kappa Light Chain

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp kappa Light Chain est conjugé à/à la CF®488A

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HP6053 L1C1

Détail du produit anti-kappa Light Chain anticorps (CF®488A)

Fonction: Mouse Monoclonal anti-Human Kappa Light Chain (HP6053 + L1C1), CF488A Conjugate

Attributs du produit: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Purified human Ig kappa chain (HP6053) & Human B-Lymphoma Cells (L1C1)

Isotype: IgG

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 0.5-1.0 μg/mL
• Flow Cytometry 0.5-2.0 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

293T, Raji or hPBL cells. Tonsil or Spleen

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur kappa Light Chain

Antigène: kappa Light Chain

Sujet: HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM

Poids moléculaire: ~22.5 kDa

ID gène: 3514, 449609