CNN1 anticorps (CF®568)

N° du produit ABIN6178188

Cet anticorps Souris Monoclonal détecte spécifiquement CNN1 dans FACS, IF et IHC (f). Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour CNN1 anticorps (CF®568) (ABIN6178188)

Antigène: CNN1 (Calponin 1 (CNN1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CNN1 est conjugé à/à la CF®568

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CALP CNN1-832

Détail du produit anti-CNN1 anticorps (CF®568)

Fonction: Mouse Monoclonal anti-Calponin-1 (CALP + CNN1/832), CF568 Conjugate

Attributs du produit: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human CNN1 protein (CNN1/832), Crude human uterus extract (CALP)

Isotype: IgG

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Myoepithelial cells in breast ducts or uterus

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur CNN1

Antigène: CNN1 (Calponin 1 (CNN1))

Autre désignation: Calponin-1

Sujet: Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC

Poids moléculaire: 34 kDa

ID gène: 1264, 465929

UniProt: P51911