KRT8 anticorps (CF®640R)

N° du produit ABIN6178862

Cet anticorps anti-KRT8 est un anticorps Souris Monoclonal détectant KRT8 dans IF, FACS et IHC (f). Adapté pour Humain, Boeuf (Vache), Lapin, Porc et Mouton.

Aperçu rapide pour KRT8 anticorps (CF®640R) (ABIN6178862)

Antigène: KRT8 (Keratin 8 (KRT8))

Reactivité: Humain, Boeuf (Vache), Lapin, Porc, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT8 est conjugé à/à la CF®640R

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C-43

Détail du produit anti-KRT8 anticorps (CF®640R)

Fonction: Mouse Monoclonal anti-Cytokeratin 8 (C-43), CF640R Conjugate

Attributs du produit: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (?ring-like, perinuclear?) from ductal (?peripheral-predominant?) carcinoma of the breast. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Cytoskeletal preparation from HeLa cells

Isotype: IgG1 kappa

Information d'application

Indications d'application: Does not react with mouse, rat, chicken or Xenopus

• Immunohistology formalin-fixed 0.5-1.0 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur KRT8

Antigène: KRT8 (Keratin 8 (KRT8))

Autre désignation: Cytokeratin 8

Sujet: CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8

Poids moléculaire: 52.5 kDa

ID gène: 3856

UniProt: P05787