Filaggrin anticorps (AA 998-1104) (CF®640R)

N° du produit ABIN6179698

L’anticorps Souris Monoclonal anti-Filaggrin a été validé pour IHC, IF, FACS et ISt. Il convient pour détecter Filaggrin dans des échantillons de Humain.

Aperçu rapide pour Filaggrin anticorps (AA 998-1104) (CF®640R) (ABIN6179698)

Antigène: Filaggrin (FLG)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Filaggrin est conjugé à/à la CF®640R

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Clone: FLG-1561

Détail du produit anti-Filaggrin anticorps (CF®640R)

Épitope: AA 998-1104

Fonction: Mouse Monoclonal anti-Filaggrin (FLG/1561), CF640R Conjugate

Attributs du produit: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human Filaggrin protein fragment (aa 998-1104) (exact sequence is proprietary)

Isotype: IgG1

Information d'application

Indications d'application: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer  pH 6.0for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

U-251-MG cells or Skin

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Filaggrin

Antigène: Filaggrin (FLG)

Autre désignation: Filaggrin

Sujet: ATOD2, Epidermal Filaggrin, Filaggrin, Filaggrin precursor, Fillagrin, FLG, Profilaggrin

Poids moléculaire: 26-45 kDa (Processed), 350 kDa (Profilaggrin)

ID gène: 2312, 654510

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance