GFAP anticorps (CF®594)

N° du produit ABIN6180668

Cet anticorps anti-GFAP Monoclonal Souris conjugué à CF®594 (Clone GA-5) (ABIN6180668) détecte spécifiquement GFAP dans WB, IF, FACS et IHC (f). L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Boeuf (Vache), Porc, Poulet et Lapin.

Aperçu rapide pour GFAP anticorps (CF®594) (ABIN6180668)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Porc, Poulet, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est conjugé à/à la CF®594

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: GA-5

Détail du produit anti-GFAP anticorps (CF®594)

Fonction: Mouse Monoclonal anti-GFAP (GA-5), CF594 Conjugate

Attributs du produit: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: GFAP isolated from pig spinal cord

Isotype: IgG1

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Brain or Astrocytoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Poids moléculaire: ~50 kDa

ID gène: 2670, 514227

UniProt: P14136