Recombinant CD15 anticorps (CF®647)

N° du produit ABIN6183844

L’anticorps Lapin Monoclonal anti-CD15 a été validé pour FACS, IHC et IF. Il convient pour détecter CD15 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Recombinant CD15 anticorps (CF®647) (ABIN6183844)

Antigène: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD15 est conjugé à/à la CF®647

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: FUT4-1478R

Détail du produit anti-CD15 anticorps (CF®647)

Fonction: Recombinant Rabbit Monoclonal anti-CD15 / FUT4 (FUT4/1478R), CF647 Conjugate

Attributs du produit: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95 % of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Purified human neutrophils

Isotype: IgG kappa

Information d'application

Indications d'application: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Flow cytometry 2-4 μg/million cells
• Immunofluorescence 2-4 μg/mL
• Immunohistology (formalin) 5-10 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

U937 cells, Reed-Sternberg',s cells in Hodgkin',s lymphoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur CD15

Antigène: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Autre désignation: CD15 / FUT4

Poids moléculaire: 220 kDa

ID gène: 2526, 654379

UniProt: P22083