MUC1 anticorps (CF®488A)

N° du produit ABIN6184124

Cet anticorps anti-MUC1 est un anticorps Souris Monoclonal détectant MUC1 dans ELISA, FACS, IF, IHC (f) et Coat. Adapté pour Humain et Souris.

Aperçu rapide pour MUC1 anticorps (CF®488A) (ABIN6184124)

Antigène: MUC1 (Mucin 1 (MUC1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC1 est conjugé à/à la CF®488A

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: 139H2

Détail du produit anti-MUC1 anticorps (CF®488A)

Fonction: Mouse Monoclonal anti-Mucin 1 / EMA / Episialin / CD227 (139H2), CF488A Conjugate

Attributs du produit: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human milk-fat globule membranes (HMFGM)

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 1-2 μg/mL
• ELISA For coating use Ab at 2-5 μg/mL, order Ab without BSA
• Flow Cytometry 0.25-0.5 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: Mucin 1 / EMA / Episialin / CD227

Poids moléculaire: 265-400 kDa

ID gène: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling