MUC1 anticorps (Biotin)

N° du produit ABIN6184169

Cet anticorps anti-MUC1 est un anticorps Souris Monoclonal détectant MUC1 dans FACS, IF et IHC (f). Adapté pour Humain.

Aperçu rapide pour MUC1 anticorps (Biotin) (ABIN6184169)

Antigène: MUC1 (Mucin 1 (MUC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC1 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: E29

Détail du produit anti-MUC1 anticorps (Biotin)

Fonction: Mouse Monoclonal anti-Mucin 1 / EMA / Episialin / CD227 (E29), Biotin Conjugate

Attributs du produit: In Western blotting, this antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Delipidated extract of human milk fat globule membranes

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: Mucin 1 / EMA / Episialin / CD227

Poids moléculaire: 265-400 kDa

ID gène: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling