Recombinant CD235a/GYPA anticorps (CF®647)

N° du produit ABIN6184756

L’anticorps Lapin Monoclonal anti-CD235a/GYPA a été validé pour FACS, IHC et IF. Il convient pour détecter CD235a/GYPA dans des échantillons de Humain.

Aperçu rapide pour Recombinant CD235a/GYPA anticorps (CF®647) (ABIN6184756)

Antigène: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD235a/GYPA est conjugé à/à la CF®647

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: GYPA-1725R

Détail du produit anti-CD235a/GYPA anticorps (CF®647)

Fonction: Recombinant Rabbit Monoclonal anti-Glycophorin-A / CD235a (GYPA/1725R), CF647 Conjugate

Attributs du produit: This antibody recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length Glycophorin A protein

Isotype: IgG kappa

Information d'application

Indications d'application: Immunohistology (formalin) 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Erythrocytes in any human tissues

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur CD235a/GYPA

Antigène: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Autre désignation: Glycophorin-A / CD235a

Sujet: Blood group--MN locus, GPA, GPErik, GpMiIII, GPSAT, GYPA, MN sialoglycoprotein, MNS, PAS2, Sialoglycoprotein alpha

Poids moléculaire: 39 kDa

ID gène: 2993, 2994

UniProt: P02724

Pathways: Maintenance of Protein Location