TDP2 anticorps (CF®405S)
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- Antigène Voir toutes TDP2 Anticorps
- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TDP2 est conjugé à/à la CF®405S
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Fonction
- Mouse Monoclonal anti-TDP2 / EAPII (TDP2/1258), CF405S Conjugate
- Attributs du produit
- This MAb recognizes a protein of 41 kDa, which is identified as TDP2. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant human full-length TDP2 protein
- Clone
- TDP2-1258
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TDP2 Anticorps primaire
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- Indications d'application
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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Jurkat cells. Lung, Duodenum or Small Intestinal Carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
- Autre désignation
- TDP2 / EAPII (TDP2 Produits)
- Synonymes
- anticorps AD022, anticorps EAP2, anticorps EAPII, anticorps TTRAP, anticorps dJ30M3.3, anticorps hTDP2, anticorps ad022, anticorps eap2, anticorps ttrap, anticorps D13Ertd656e, anticorps Ttrap, anticorps tdp2, anticorps ttrapl, anticorps tyrosyl-DNA phosphodiesterase 2, anticorps tyrosyl-DNA phosphodiesterase 2 S homeolog, anticorps tyrosyl-DNA phosphodiesterase 2b, anticorps TDP2, anticorps tdp2.S, anticorps Tdp2, anticorps tdp2b
- Poids moléculaire
- ~41 kDa
- ID gène
- 51567
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