MAML3 anticorps (CF®405S)

N° du produit ABIN6188130

Cet anticorps Souris Monoclonal détecte spécifiquement MAML3 dans FACS, IF et ISt. Il présente une réactivité envers Humain.

Aperçu rapide pour MAML3 anticorps (CF®405S) (ABIN6188130)

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAML3 est conjugé à/à la CF®405S

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt)

Clone: MAML3-1303

Détail du produit anti-MAML3 anticorps (CF®405S)

Fonction: Mouse Monoclonal anti-MAML3 (MAML3/1303), CF405S Conjugate

Attributs du produit: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J?, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length MAML3 protein

Isotype: IgG1

Information d'application

Indications d'application: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur MAML3

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Autre désignation: MAML3

Sujet: CAGH3, ERDA3, GDN, MAML3, Mastermind-like protein 3, TNRC3

Poids moléculaire: 150-170 kDa

ID gène: 55534