pan Keratin anticorps (CF®647)

N° du produit ABIN6190228

Cet anticorps anti-pan Keratin est un anticorps Souris Monoclonal détectant pan Keratin dans IF, FACS, WB et IHC (f). Adapté pour Humain, Rat, Souris, Boeuf (Vache), Singe, Chien, Lapin et Poulet.

Aperçu rapide pour pan Keratin anticorps (CF®647) (ABIN6190228)

Antigène: pan Keratin (panKRT)

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Singe, Chien, Lapin, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp pan Keratin est conjugé à/à la CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: AE-1-AE-3

Détail du produit anti-pan Keratin anticorps (CF®647)

Fonction: Mouse Monoclonal anti-Cytokeratin, pan (AE-1 / AE-3), CF647 Conjugate

Attributs du produit: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human epidermal keratin

Isotype: IgG

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Skin, Adeno- or Squamous carcinomas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Cytokeratin, pan

Sujet: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Poids moléculaire: 40-67 kDa

ID gène: 374454, 51350