Nuclear Antigen anticorps

N° du produit ABIN6190827

Détail du produit

Antigène: Nuclear Antigen

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Fonction: Mouse Monoclonal anti-Human Nuclear Antigen (235-1), Purified, BSA-free

Attributs du produit: This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Nuclei of human myeloid leukemia biopsy cells

Clone: 235-1

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology Frozen 0.5-1.0 μg/mL

• Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Does not react with mouse, rat or chicken, others not known
• Optimal dilution for a specific application should be determined by user

Commentaires:

All human cells. Tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détail du antigène

Antigène: Nuclear Antigen

Sujet: HNA, HuNu

Poids moléculaire: 70 kDa & 80 kDa