ACTH anticorps (N-Term)

N° du produit ABIN6190941

Détail du produit anti-ACTH anticorps

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Épitope: N-Term

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACTH est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Coating (Coat)

Fonction: Mouse Monoclonal anti-ACTH (57), Purified, BSA-free

Attributs du produit: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: N-terminal fragment of human ACTH conjugated to KLH

Clone: 57

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• ELISA For coating, order Ab without BSA
• Predicted to show broad species reactivity
• Optimal dilution for a specific application should be determined by user

Commentaires:

Normal pituitary gland or pituitary tumor

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur ACTH

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Autre désignation: ACTH (Adrenocorticotrophic Hormone) (ACTH Produits)

Synonymes: anticorps ACTH, anticorps BE, anticorps Beta-LPH, anticorps Clip, anticorps Gamma-LPH, anticorps Npp, anticorps Pomc-1, anticorps Pomc1, anticorps alpha-MSH, anticorps alphaMSH, anticorps beta-MSH, anticorps gamma-MSH, anticorps CLIP, anticorps LPH, anticorps MSH, anticorps NPP, anticorps POC, anticorps pro-opiomelanocortin-alpha, anticorps proopiomelanocortin, anticorps Pomc, anticorps POMC

Classe de substances: Hormone

Sujet: Adrenocorticotropin, alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta-Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC

Poids moléculaire: ACTH is ~5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

ID gène: 5443, 1897

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity