Recombinant ACTH anticorps (N-Term) (CF®594)

N° du produit ABIN6191156

Cet anticorps Souris Monoclonal détecte spécifiquement ACTH dans IHC, IF, FACS, ELISA et Coat. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour Recombinant ACTH anticorps (N-Term) (CF®594) (ABIN6191156)

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACTH est conjugé à/à la CF®594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Coating (Coat)

Clone: R57

Détail du produit anti-ACTH anticorps (CF®594)

Épitope: N-Term

Fonction: Recombinant Mouse Monoclonal anti-ACTH (r57), CF594 Conjugate

Attributs du produit: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), it does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: N-terminal fragment of human ACTH conjugated to KLH

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Commentaires:

Normal pituitary gland or pituitary tumor

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur ACTH

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Autre désignation: ACTH

Classe de substances: Hormone

Sujet: Adrenocorticotropin, alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta-Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC

Poids moléculaire: ACTH is ~5 kDa

ID gène: 5443, 1897

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity