TUBB anticorps (Chain beta)
Aperçu rapide pour TUBB anticorps (Chain beta) (ABIN619536)
Antigène
Voir toutes TUBB AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- Chain beta
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Fonction
- Anti-Tubulin beta chain
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Réactivité croisée (Details)
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Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
Not reactive in: Allium sativum -
Homologie
- Brassica napus,Chlamydomonas reinhardii,Glycine max,Hordeum vulgare,Micromonoas pusilla,Nicotiana attenuata,Oryza sativa,Ostreococcus lucimarinus,Picea sitcHensis,Pisum sativum,Physcomitrium patens,Populus trichocarpa,Pythium ultimum,Saccharomyces cerevisiae,Solanum tuberosum,Sorghum bicolor,Ricinus communis,Zea mays,Vigna radiata,Vitis vinifera
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Purification
- Immunogen affinity purified serum
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Immunogène
- KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512
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Indications d'application
- 1 : 1000 (IF), 1 : 500 (WB)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Conseil sur la manipulation
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Stock
- -20 °C
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Stockage commentaire
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- TUBB (Tubulin, beta (TUBB))
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Autre désignation
- Tubulin beta chain
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Sujet
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Background: Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
Additional information: Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 μg/well and a dilution of 1: 500.
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Poids moléculaire
- 49 | 49 kDa (Arabidopsis thaliana)
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ID gène
- 836390
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UniProt
- P29512, P24636, P29513, P29514, P29515, P29516, P29517
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Pathways
- Dynamique des Microtubules, M Phase
Antigène
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