HSP18.5 anticorps

N° du produit ABIN619791

Détail du produit anti-HSP18.5 anticorps

Antigène: HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Homologie: dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 18.5 kDa

Purification: serum

Immunogène: recombinant Arabidopsis thaliana Hsp18.5

Information d'application

Indications d'application: 1 : 1000 with standard ECL (WB)

Commentaires:

hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Détails sur HSP18.5

Antigène: HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))

Autre désignation: HSP18.5

Sujet: HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein

Poids moléculaire: 18.5 kDa