Nucleoli Marker anticorps

N° du produit ABIN6251821

L’anticorps anti-Nucleoli Marker Monoclonal Souris est utilisé pour la détection de Nucleoli Marker dans des échantillons de Humain. Il a été validé pour IF, FACS, IHC et ICC.

Aperçu rapide pour Nucleoli Marker anticorps (ABIN6251821)

Antigène: Nucleoli Marker

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: NCM102-1

Détail du produit

Purification: Protein G affinity chromatography

Immunogène: A preparation of myeloid leukemia cell nuclei was used as the immunogen.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 0.5-1.0 μg/mL

Immunocytochemistry (Acetone-fixed): 0.5-1.0 μg/mL for 30 min at RT

IHC (Formalin-paraffin): 0.5-1.0 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the Nucleoli marker antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détail du antigène

Antigène: Nucleoli Marker

Sujet: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]