POMC anticorps (N-Term)

N° du produit ABIN6251838

Cet anticorps Souris Monoclonal détecte spécifiquement POMC dans WB, IHC, FACS et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour POMC anticorps (N-Term) (ABIN6251838)

Antigène: POMC (Proopiomelanocortin (POMC))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POMC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 57

Détail du produit anti-POMC anticorps

Épitope: N-Term

Purification: Protein G affinity chromatography

Immunogène: An N-terminal peptide from the ACTH region of human POMC was used as the immunogen for this POMC antibody.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

The optimal dilution of the ACTH antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur POMC

Antigène: POMC (Proopiomelanocortin (POMC))

Autre désignation: POMC

Sujet: POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.

ID gène: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour