CD99 anticorps

N° du produit ABIN6251917

L’anticorps Souris Monoclonal anti-CD99 a été validé pour FACS, WB, IF et IHC (p). Il convient pour détecter CD99 dans des échantillons de Humain et Rat.

Aperçu rapide pour CD99 anticorps (ABIN6251917)

Antigène: CD99

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD99 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HO36-1-1

Détail du produit anti-CD99 anticorps

Purification: PEG precipitation

Immunogène: Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Western blot: 1-2 μg/mL

Flow Cytometry: 5-10 μL/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the CD99 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD99

Antigène: CD99

Autre désignation: CD99

Sujet: CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]

ID gène: 4267

UniProt: P14209