Actin, Muscle Specific anticorps

N° du produit ABIN6251982

Cet anticorps Souris Monoclonal détecte spécifiquement Actin, Muscle Specific dans FACS, IF et IHC. Il présente une réactivité avec des échantillons de Humain, Lapin et Rat.

Aperçu rapide pour Actin, Muscle Specific anticorps (ABIN6251982)

Antigène: Actin, Muscle Specific

Reactivité: Humain, Lapin, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin, Muscle Specific est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: SPM160

Détail du produit anti-Actin, Muscle Specific anticorps

Purification: Protein G affinity chromatography

Immunogène: Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Actin, Muscle Specific

Antigène: Actin, Muscle Specific

Autre désignation: Muscle Specific Actin

Sujet: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

ID gène: 59

UniProt: P62736