FOXA1 anticorps (AA 372-472)

N° du produit ABIN6251998

L’anticorps anti-FOXA1 Monoclonal Souris est utilisé pour la détection de FOXA1 dans des échantillons de Humain. Il a été validé pour WB, IHC, FACS et IF.

Aperçu rapide pour FOXA1 anticorps (AA 372-472) (ABIN6251998)

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FOXA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: FOXA1-1514

Détail du produit anti-FOXA1 anticorps

Épitope: AA 372-472

Specificité: Does not react with rat

Purification: Protein G affinity chromatography

Immunogène: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur FOXA1

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Autre désignation: FOXA1

Sujet: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

ID gène: 3169

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis