Ferritin anticorps (AA 38-165)

N° du produit ABIN6252003

Cet anticorps Souris Monoclonal détecte spécifiquement Ferritin dans WB, IHC, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour Ferritin anticorps (AA 38-165) (ABIN6252003)

Antigène: Ferritin (FE)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ferritin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FTLC1-1

Détail du produit anti-Ferritin anticorps

Épitope: AA 38-165

Purification: Protein G affinity chromatography

Immunogène: Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Ferritin

Antigène: Ferritin (FE)

Autre désignation: Ferritin

Sujet: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

ID gène: 2512

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis