Involucrin anticorps (AA 421-568)

N° du produit ABIN6252058

L’anticorps Souris Monoclonal anti-Involucrin a été validé pour IHC, IF et FACS. Il convient pour détecter Involucrin dans des échantillons de Humain, Chien, Porc, Gorilla et Owl Monkey.

Aperçu rapide pour Involucrin anticorps (AA 421-568) (ABIN6252058)

Antigène: Involucrin (IVL)

Reactivité: Humain, Chien, Porc, Gorilla, Owl Monkey

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM259

Détail du produit anti-Involucrin anticorps

Épitope: AA 421-568

Specificité: Does not react with Mouse

Purification: Protein G affinity chromatography

Immunogène: Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin antibody. Its epitope maps between amino acids 421-568 of human involucrin.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the Involucrin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: Involucrin

Sujet: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

ID gène: 3713

UniProt: P07476