TTF1 anticorps

N° du produit ABIN6252067

L’anticorps Souris Monoclonal anti-TTF1 a été validé pour FACS, IHC (p) et IF. Il convient pour détecter TTF1 dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour TTF1 anticorps (ABIN6252067)

Antigène: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TTF1 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: HBNK2-1

Détail du produit anti-TTF1 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant protein was used as the immunogen for the TTF1 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the TTF1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur TTF1

Antigène: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Autre désignation: TTF1

Sujet: Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.

ID gène: 7080

UniProt: P43699

Pathways: Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour