Vimentin anticorps

N° du produit ABIN6252111

Cet anticorps Souris Monoclonal détecte spécifiquement Vimentin dans WB, IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat et Chévre.

Aperçu rapide pour Vimentin anticorps (ABIN6252111)

Antigène: Vimentin (VIM)

Reactivité: Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM576

Détail du produit anti-Vimentin anticorps

Specificité: Does not react with mouse and rat

Purification: Protein G affinity chromatography

Immunogène: Human recombinant protein was used as the immunogen for this VIM antibody.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Western blot: 0.25-0.5 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the VIM antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: VIM

Sujet: This mAb reacts with a 58  kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis