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VEGF anticorps

Cet anticorps anti-VEGF est un anticorps Souris Monoclonal détectant VEGF dans WB, IHC (p) et IF. Adapté pour Humain, Souris, Rat, Chien et Lapin.
N° du produit ABIN6252287

Aperçu rapide pour VEGF anticorps (ABIN6252287)

Antigène

Voir toutes VEGF Anticorps
VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivité

  • 85
  • 30
  • 19
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Humain, Souris, Rat, Chien, Lapin

Hôte

  • 43
  • 42
  • 14
  • 2
  • 2
  • 2
Souris

Clonalité

  • 52
  • 50
  • 1
Monoclonal

Conjugué

  • 47
  • 17
  • 6
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp VEGF est non-conjugé

Application

  • 77
  • 59
  • 39
  • 24
  • 20
  • 15
  • 10
  • 7
  • 6
  • 6
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone

VEGF-1063
  • Purification

    Protein G affinity chromatography

    Immunogène

    Human recombinant protein was used as the immunogen for the VEGF antibody.

    Isotype

    IgG1 kappa
  • Indications d'application

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

    Optimal dilution of the VEGF antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.2 mg/mL

    Buffer

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    -20 °C

    Stockage commentaire

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Antigène

    VEGF (Vascular Endothelial Growth Factor (VEGF))

    Autre désignation

    VEGF

    Sujet

    This mAb recognizes proteins of 19-22  kDa (reducing) and 38  kDa-44  kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

    ID gène

    7422

    UniProt

    P15692
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