CD45RA anticorps

N° du produit ABIN6252424

L’anticorps Souris Monoclonal anti-CD45RA a été validé pour FACS, IF et IHC. Il convient pour détecter CD45RA dans des échantillons de Humain.

Aperçu rapide pour CD45RA anticorps (ABIN6252424)

Antigène: CD45RA

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: 111-1C5

Détail du produit anti-CD45RA anticorps

Purification: Protein G affinity chromatography

Immunogène: Stimulated human leukocytes were used as immunogen for this CD45RA antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

Immunofluorescence: 1-2 μg/mL

Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD45RA

Antigène: CD45RA

Autre désignation: CD45RA

Sujet: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

ID gène: 5788

UniProt: P08575