ECM1 anticorps

N° du produit ABIN6252531

Cet anticorps Souris Monoclonal détecte spécifiquement ECM1 dans IHC et IF. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour ECM1 anticorps (ABIN6252531)

Antigène: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ECM1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: SC05

Détail du produit anti-ECM1 anticorps

Purification: Protein G affinity chromatography

Immunogène: Secretory component protein isolated from human colostrum was used as the immunogen.

Isotype: IgG1 kappa

Information d'application

Indications d'application: IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur ECM1

Antigène: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Autre désignation: Extracellular Matrix Protein 1

Sujet: This antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory component. It does not react with the cell lines lacking this protein. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

ID gène: 1893

UniProt: Q16610