HCG beta anticorps

N° du produit ABIN6252542

Cet anticorps anti-HCG beta est un anticorps Souris Monoclonal détectant HCG beta dans ELISA, WB et IHC (fro). Adapté pour Humain.

Aperçu rapide pour HCG beta anticorps (ABIN6252542)

Antigène: HCG beta

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HCG beta est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HCGb-211

Détail du produit anti-HCG beta anticorps

Purification: Protein G affinity chromatography

Immunogène: Purified HCG-beta protein was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: ELISA: use BSA-free format (1)

IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.

1. Clone HCGb/211 (recommended capture) will pair with clones < a href=../tds/hcg-beta-antibody-hcgb459-v2373>HCGb/459 and < a href=../tds/hcg-beta-antibody-hcgb54-v2092>HCGb/54 (recommended detect) by ELISA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur HCG beta

Antigène: HCG beta

Autre désignation: HCG-beta

Sujet: This antibody reacts with a protein of 22  kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

ID gène: 1082

UniProt: Q6NT52