CD5 anticorps

N° du produit ABIN6252678

Cet anticorps Souris Monoclonal détecte spécifiquement CD5 dans FACS, WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Rat, Boeuf (Vache), Lapin, Cheval, Porc, Singe, Poulet et Cobaye.

Aperçu rapide pour CD5 anticorps (ABIN6252678)

Antigène: CD5

Reactivité: Humain, Rat, Boeuf (Vache), Lapin, Cheval, Porc, Singe, Poulet, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: CD5-54-F6

Détail du produit anti-CD5 anticorps

Purification: Protein G affinity chromatography

Immunogène: A peptide from the intracellular region of the human protein was used as the immunogen for this CD5 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the CD5 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD5

Antigène: CD5

Autre désignation: CD5

Sujet: Recognizes a 67  kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

ID gène: 921

UniProt: P06127