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Igk anticorps

Igk Reactivité: Humain FACS, IHC, WB, IF Hôte: Souris Monoclonal SPM558 unconjugated
N° du produit ABIN6252720
  • Antigène Tous les produits Igk
    Igk (Immunoglobulin kappa Chain Complex (Igk))
    Reactivité
    • 5
    • 1
    Humain
    Hôte
    • 4
    • 1
    • 1
    Souris
    Clonalité
    • 4
    • 2
    Monoclonal
    Conjugué
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Igk est non-conjugé
    Application
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
    Purification
    Protein G affinity chromatography
    Immunogène
    Recombinant human Ig kappa chain was used as the immunogen for this anti-Kappa antibody.
    Clone
    SPM558
    Isotype
    IgG1 kappa
  • Indications d'application
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Western blot: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    The optimal dilution of the anti-Kappa antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Antigène
    Igk (Immunoglobulin kappa Chain Complex (Igk))
    Autre désignation
    Kappa (Igk Produits)
    Synonymes
    anticorps kappa, anticorps immunoglobulin kappa chain complex, anticorps Igk
    Sujet
    This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    ID gène
    3514
    UniProt
    P01601, P01834
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