MAML3 anticorps

N° du produit ABIN6252771

Cet anticorps Souris Monoclonal détecte spécifiquement MAML3 dans FACS, IF et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour MAML3 anticorps (ABIN6252771)

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAML3 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: MMLP3-1

Détail du produit anti-MAML3 anticorps

Purification: Protein G affinity chromatography

Immunogène: A human full length recombinant protein was used as the immunogen for this MAML3 antibody.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur MAML3

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Autre désignation: MAML3

Sujet: Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.

ID gène: 55534

UniProt: Q96JK9