TIMP3 anticorps (AA 175-211)

N° du produit ABIN6252786

Cet anticorps Lapin Polyclonal détecte spécifiquement TIMP3 dans WB, IHC, IP, FACS et IF. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chien et Cheval.

Aperçu rapide pour TIMP3 anticorps (AA 175-211) (ABIN6252786)

Antigène: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TIMP3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Détail du produit anti-TIMP3 anticorps

Épitope: AA 175-211

Purification: Protein A affinity chromatography

Immunogène: Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry: use antibody at 0.5-1 μg/million cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IP: 1-2 μg/500 μg protein lysate

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur TIMP3

Antigène: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Autre désignation: TIMP3

Sujet: TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

ID gène: 7078

UniProt: P35625