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Secretory Component Glycoprotein anticorps

Cet anticorps anti-Secretory Component Glycoprotein est un anticorps Souris Monoclonal détectant Secretory Component Glycoprotein dans IHC (p), IF, FACS et WB. Adapté pour Humain et Rat.
N° du produit ABIN6252815

Aperçu rapide pour Secretory Component Glycoprotein anticorps (ABIN6252815)

Antigène

Secretory Component Glycoprotein

Reactivité

Humain, Rat

Hôte

  • 3
Souris

Clonalité

  • 3
Monoclonal

Conjugué

  • 3
Cet anticorp Secretory Component Glycoprotein est non-conjugé

Application

  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Clone

ECM1-792
  • Purification

    Protein G affinity chromatography

    Immunogène

    Recombinant human protein was used as the immunogen for the Secretory Component Glycoprotein antibody.

    Isotype

    IgG1 kappa
  • Indications d'application

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Optimal dilution of the Secretory Component Glycoprotein antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.2 mg/mL

    Buffer

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    -20 °C

    Stockage commentaire

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Antigène

    Secretory Component Glycoprotein

    Sujet

    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

    ID gène

    1893

    UniProt

    Q16610
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