pan Keratin anticorps

N° du produit ABIN6252866

Cet anticorps anti-pan Keratin est un anticorps Lapin Polyclonal détectant pan Keratin dans IF, FACS, WB et IHC (p). Adapté pour Humain.

Aperçu rapide pour pan Keratin anticorps (ABIN6252866)

Antigène: pan Keratin (panKRT)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp pan Keratin est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-pan Keratin anticorps

Purification: Protein A affinity chromatography

Immunogène: Recombinant full-length human KRT76 and KRT77 proteins were used as the immunogen for this pan Cytokeratin antibody.

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10e6 cells in 0.1ml

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT

Prediluted format: incubate for 30 min at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Pan Cytokeratin

Sujet: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody detects acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins: 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8), 56.5  kDa (CK10), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), 40  kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.

ID gène: 3848, 3850, 3851

UniProt: P04264, P12035, P19013, P13647, P48668, P05787, P13645, P02533, P19012, P08779, P08727