ACTH anticorps (AA 25-39)
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- Antigène Voir toutes ACTH Anticorps
- ACTH (Adrenocorticotropic hormone (ACTH))
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Épitope
- AA 25-39
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ACTH est non-conjugé
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone (CLIP sequence) were used as the immunogen for this ACTH antibody.
- Clone
- CLIP-1407
- Isotype
- IgG1
- Top Product
- Discover our top product ACTH Anticorps primaire
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- Indications d'application
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IHC (FFPE): 0.5-1 μg/mL
ELISA: order BSA free format for coating
Flow Cytometry: 0.5-1 μg/10^6 cells
IF: 1-2 μg/mL
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Antigène
- ACTH (Adrenocorticotropic hormone (ACTH))
- Autre désignation
- ACTH (ACTH Produits)
- Synonymes
- anticorps ACTH, anticorps BE, anticorps Beta-LPH, anticorps Clip, anticorps Gamma-LPH, anticorps Npp, anticorps Pomc-1, anticorps Pomc1, anticorps alpha-MSH, anticorps alphaMSH, anticorps beta-MSH, anticorps gamma-MSH, anticorps CLIP, anticorps LPH, anticorps MSH, anticorps NPP, anticorps POC, anticorps pro-opiomelanocortin-alpha, anticorps proopiomelanocortin, anticorps Pomc, anticorps POMC
- Classe de substances
- Hormone
- Sujet
- ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
- ID gène
- 5443
- UniProt
- P01189
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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