SMN1 anticorps

N° du produit ABIN6253498

Cet anticorps anti-SMN1 est un anticorps Souris Monoclonal détectant SMN1 dans WB et IP. Adapté pour Humain.

Aperçu rapide pour SMN1 anticorps (ABIN6253498)

Antigène: SMN1 (Survival of Motor Neuron 1, Telomeric (SMN1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SMN1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 7B10

Détail du produit anti-SMN1 anticorps

Fonction: anti-Survival Motor Neuron Protein (human), mAb (7B10)

Attributs du produit: Monoclonal Antibody. Recognizes a sequence of up to 9 amino acids in the N-terminal domain of human SMN. Isotype: Mouse IgG1. Clone: 7B10. Applications: IP, WB. Liquid. In PBS. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs. It is expressed in a wide variety of tissues, including high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle and low levels in fibroblasts and lymphocytes. Mutations in SMN cause spinal muscular atrophy 1 (SMA1). Spinal muscular atrophy is a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. The antibody was used extensively to purify and characterize the macromolecular SMN complex from cultured human cells. The antibody binds with high affinity and specificity to its epitope, which comprises a sequence of up to 9 amino acids in the N-terminal part of human SMN. It allows the native elution of bound antigen with an excess of the cognate peptide. Via addition of the 7B10 epitope (TagIt epitope) to other proteins this purification strategy can also be exploited for purification of these fusion proteins and RNA-protein complexes from cells lacking this epitope on endogenous proteins, such as cells derived from C. elegans and Drosophila.

Purification: Protein A/G purified.

Immunogène: Recombinant human full-length survival motor neuron protein (SMN).

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. In PBS.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

Détails sur SMN1

Antigène: SMN1 (Survival of Motor Neuron 1, Telomeric (SMN1))

Autre désignation: Survival Motor Neuron Protein

Sujet:

Alternate Names/Synonyms: SMN, Gemin-1, Component of Gems 1, BCD541

Product Description: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs. It is expressed in a wide variety of tissues, including high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle and low levels in fibroblasts and lymphocytes. Mutations in SMN cause spinal muscular atrophy 1 (SMA1). Spinal muscular atrophy is a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. The antibody was used extensively to purify and characterize the macromolecular SMN complex from cultured human cells. The antibody binds with high affinity and specificity to its epitope, which comprises a sequence of up to 9 amino acids in the N-terminal part of human SMN. It allows the native elution of bound antigen with an excess of the cognate peptide. Via addition of the 7B10 epitope (TagIt epitope) to other proteins this purification strategy can also be exploited for purification of these fusion proteins and RNA-protein complexes from cells lacking this epitope on endogenous proteins, such as cells derived from C. elegans and Drosophila.

UniProt: Q16637

Pathways: Ribonucleoprotein Complex Subunit Organization