tdTomato anticorps

N° du produit ABIN6254170

Cet anticorps Chèvre Polyclonal détecte spécifiquement tdTomato dans IF, IHC (fro), WB, IEM et IHC (p). Il présente une réactivité avec des échantillons de Tag. Il a été cité dans 12+ publications.

Aperçu rapide pour tdTomato anticorps (ABIN6254170)

Antigène: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reactivité: Tag

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp tdTomato est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-tdTomato anticorps

Fonction: anti-tdTomato

Séquence: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Specificité: In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Réactivité croisée (Details): tdTomato, mCherry, RFP

Attributs du produit: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogène: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Restrictions: For Research Use only

Stockage

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody solution should be gently mixed before use.

Stock: 4 °C,-20 °C

Stockage commentaire: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Références pour anticorps anti-tdTomato (ABIN6254170)

Saavedra, Wallace, Freudenrich, Mall, Mundy, Davila, Shafer, Wernig, Haag: "Comparison of Acute Effects of Neurotoxic Compounds on Network Activity in Human and Rodent Neural Cultures." dans: Toxicological sciences : an official journal of the Society of Toxicology, (2021) (PubMed).

Cadwell, Scala, Fahey, Kobak, Mulherkar, Sinz, Papadopoulos, Tan, Johnsson, Hartmanis, Li, Cotton, Tolias, Sandberg, Berens, Jiang, Tolias: "Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex." dans: eLife, Vol. 9, (2020) (PubMed).

Unnersjö-Jess, Scott, Sevilla, Patrakka, Blom, Brismar: "Confocal super-resolution imaging of the glomerular filtration barrier enabled by tissue expansion." dans: Kidney international, Vol. 93, Issue 4, pp. 1008-1013, (2019) (PubMed).

Park, Choi, Park, Sim, Park, Kang, Yim, Lee, Kim, Pac, Rhee, Lee, Lee, Lee, Kim: "Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio." dans: Advanced science (Weinheim, Baden-Wurttemberg, Germany), Vol. 6, Issue 22, pp. 1901673, (2019) (PubMed).

Niquille, Limoni, Markopoulos, Cadilhac, Prados, Holtmaat, Dayer: "Neurogliaform cortical interneurons derive from cells in the preoptic area." dans: eLife, Vol. 7, (2019) (PubMed).

Tai, Gallo, Wang, Yu, Van Aelst: "Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM." dans: Neuron, Vol. 102, Issue 2, pp. 358-372.e9, (2019) (PubMed).

Ishibashi, Shimizu, Nakata, Fujii, Suzuki, Kawamoto, Anzai, Kuno, Nagata, Ito, Murano, Mizutani, Oshima, Tsuchiya, Nakamura, Watanabe, Okamoto: "Contribution of ATOH1+ Cells to the Homeostasis, Repair, and Tumorigenesis of the Colonic Epithelium." dans: Stem cell reports, Vol. 10, Issue 1, pp. 27-42, (2018) (PubMed).

Paolino, Fenlon, Kozulin, Richards, Suárez: "Multiple events of gene manipulation via in pouch electroporation in a marsupial model of mammalian forebrain development." dans: Journal of neuroscience methods, Vol. 293, pp. 45-52, (2018) (PubMed).

Hurni, Kolodziejczak, Tomasello, Badia, Jacobshagen, Prados, Dayer: "Transient Cell-intrinsic Activity Regulates the Migration and Laminar Positioning of Cortical Projection Neurons." dans: Cerebral cortex (New York, N.Y. : 1991), Vol. 27, Issue 5, pp. 3052-3063, (2018) (PubMed).

Weider, Starost, Groll, Küspert, Sock, Wedel, Fröb, Schmitt, Baroti, Hartwig, Hillgärtner, Piefke, Fadler, Ehrlich, Ehlert, Stehling, Albrecht, Jabali, Schöler, Winkler, Kuhlmann, Wegner: "Nfat/calcineurin signaling promotes oligodendrocyte differentiation and myelination by transcription factor network tuning." dans: Nature communications, Vol. 9, Issue 1, pp. 899, (2018) (PubMed).

Anzo, Sekine, Makihara, Chao, Miura, Chihara: "Dendritic Eph organizes dendrodendritic segregation in discrete olfactory map formation in Drosophila." dans: Genes & development, Vol. 31, Issue 10, pp. 1054-1065, (2017) (PubMed).

Beattie, Postiglione, Burnett, Laukoter, Streicher, Pauler, Xiao, Klezovitch, Vasioukhin, Ghashghaei, Hippenmeyer: "Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells." dans: Neuron, Vol. 94, Issue 3, pp. 517-533.e3, (2017) (PubMed).

Détails sur tdTomato

Antigène: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Autre désignation: tdTomato

Sujet:

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.

Cherry fluorescent protein, DsRed, mCherry, red fluorescent protein, RFP antibody.