Cet anticorps anti-RFP est un anticorps Chèvre Polyclonal détectant RFP dans WB, IF, IHC (fro) et IHC (p). Adapté pour Discosoma. Ce Primary Antibody a été cité dans 4+ publications.
In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Réactivité croisée (Details)
Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato
Purification
epitope affinity purified
Immunogène
Purified recombinant RFP peptide produced in E. coli.
Western blot 1:500-1:2,000 Immunofluorescence 1:50-1:500 Immunohistochemistry (paraffin) 1:50-1:500 Immunohistochemistry (frozen) 1:50-1:500
Restrictions
For Research Use only
Conseil sur la manipulation
The antibody solution should be gently mixed before use.
Stock
4 °C/-20 °C
Stockage commentaire
For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.
Krishna, Choudhury, Keough, Seo, Ni, Kakaizada, Lee, Aabedi, Popova, Lipkin, Cao, Nava Gonzales, Sudharshan, Egladyous, Almeida, Zhang, Molinaro, Venkatesh, Daniel, Shamardani, Hyer, Chang, Findlay, Phillips, Nagarajan, Raleigh, Brang, Monje, Hervey-Jumpe: "Glioblastoma remodelling of human neural circuits decreases survival." dans: Nature, Vol. 617, Issue 7961, pp. 599-607, (2023) (PubMed).
Tian, Peng, Murugan, Feng, Liu, Eyo, Zhou, Mogilevsky, Wang, Wu: "Chemokine CCL2-CCR2 Signaling Induces Neuronal Cell Death via STAT3 Activation and IL-1β Production after Status Epilepticus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 33, pp. 7878-7892, (2017) (PubMed).