Abscisic Acid Insensitive 5 (ABI5) anticorps

N° du produit ABIN4966067

L’anticorps anti-Abscisic Acid Insensitive 5 (ABI5) Polyclonal Lapin est utilisé pour la détection de Abscisic Acid Insensitive 5 (ABI5) dans des échantillons de Arabidopsis thaliana. Il a été validé pour IL et WB.

Aperçu rapide pour Abscisic Acid Insensitive 5 (ABI5) anticorps (ABIN4966067)

Antigène: Abscisic Acid Insensitive 5 (ABI5)

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Détail du produit

Fonction: Anti-ABI5 | Abscisic acid insensitive 5 (peptide antibody)

Réactivité croisée (Details): Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Brassica napus,Populus trichocarpa

Purification: Immunogen affinity purified serum

Immunogène: KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence

Information d'application

Indications d'application: 1: 140 (IL), 1 : 200 (WB)

Commentaires:

ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as most sensitive detection reagents.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water to each tube

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détail du antigène

Antigène: Abscisic Acid Insensitive 5 (ABI5)

Sujet:

Background: ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.

Additional information: MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here.

Poids moléculaire: 47 kDa

ID gène: 818199

UniProt: Q9SJN0