SKP2 anticorps (pSer64)

N° du produit ABIN6255441

L’anticorps anti-SKP2 Polyclonal Lapin est utilisé pour la détection de SKP2 dans des échantillons de Humain et Souris. Il a été validé pour WB et IHC.

Aperçu rapide pour SKP2 anticorps (pSer64) (ABIN6255441)

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SKP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-SKP2 anticorps

Épitope: pSer64

Specificité: Phospho-Skp2 (Ser64) Antibody detects endogenous levels of Skp2.

Réactivité croisée: Humain, Souris

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human Skp2 around the phosphorylation site of Ser64.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C.Stable for 12 months from date of receipt

Date de péremption: 12 months

Détails sur SKP2

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Autre désignation: Skp2

Sujet:

Description: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

Gene: SKP2

Poids moléculaire: 48kDa

ID gène: 6502

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases